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Image Search Results
Journal: Cell Reports
Article Title: The Ubiquitin Ligase TRIP12 Limits PARP1 Trapping and Constrains PARP Inhibitor Efficiency
doi: 10.1016/j.celrep.2020.107985
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Modification, Protease Inhibitor, Ligation, Labeling, Purification, Plasmid Preparation, Software
Journal: Parasites & Vectors
Article Title: Phosphatase UBLCP1 is required for the growth, virulence and mitochondrial integrity of Toxoplasma gondii
doi: 10.1186/s13071-025-06766-3
Figure Lengend Snippet: Characterization of UBLCP1 in Toxoplasma gondii . A , Diagram illustrating the CRISPR–Cas9-mediated homologous recombination of T. gondii UBLCP1 at the uracil phosphoribosyl transferase (UPRT) site. The donor fragment is composed of full-length UBLCP1 fused with the EGFP epitope, controlled by a tubulin promoter. B , PCR amplification analyses of target sequence (PCR1) and 5′ and 3′ integration of homologous fragments (PCR2 and PCR3) in UBLCP1-EGFP tachyzoites. C , Western blot analysis of UBLCP1-EGFP and Tub-EGFP strains with rabbit anti-GFP antibody. D , The subcellular localization of UBLCP1-EGFP in tachyzoites of T. gondii , as determined by indirect immunofluorescence. E , Subcellular localization of UBLCP1 is restricted to the nucleus at different stages of the T. gondii tachyzoite cell cycle. Green indicates rabbit anti-GFP antibody. Red indicates IMC1. Blue indicates DNA-specific dye with DAPI. Scale bar, 2 µm
Article Snippet: The primary
Techniques: CRISPR, Homologous Recombination, Amplification, Sequencing, Western Blot, Immunofluorescence